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2022
Garmendia, María Luisa
IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study. Artículo de revista
En: Sci Rep, 2022.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_58,
title = {IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study.},
author = {María Luisa Garmendia},
doi = {10.1038/s41598-022-13063-x},
year = {2022},
date = {2022-00-00},
urldate = {2022-00-00},
journal = {Sci Rep},
abstract = {- Otros autores: Ampuero S, Bahamonde G, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Lizama L, López M, Avendaño LF, Luchsinger V.
- Cómo citar: "Ampuero S, Bahamonde G, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Lizama L, López M, Avendaño LF, Luchsinger V. IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study. Sci Rep.
2022;12(1):13145. doi: 10.1038/s41598-022-13063-x. PMID: 35907923 PMCID: PMC9339003. ISSN: 2045-2322. IF [JCR]: 4.996. Q2"
- Resumen: Community-acquired pneumonia (CAP) is a worldwide leading cause of death. Recognized risk factors in some severe cases have not been identified. Lymphocytopenia has been frequently described in CAP. Since IL-7, membrane-bound receptor (IL7Rα;CD127) and soluble IL7Rα (sIL7R) are critical in lymphocytes homeostasis, in this work we aimed to evaluate the involvement of the IL-7/IL7Rα axis in the severity of adult CAP, since it has not been explored. The IL7Rα SNPs rs6897932, rs987106, and rs3194051 SNPs in IL7α were genotyped, the systemic expression of the IL7R gene, sIL7R, IL-7, and levels of peripheral IL7Rα+ T lymphocytes were quantified in 202 hospitalized CAP cases. rs3194051GG was more frequent in non-survivors than in survivors; rs987106TT was more frequent and rs3194051AA less frequent in patients at intensive care unit (ICU) than in those not admitted to ICU. IL7Rα gene expression was lower in non-survivors than in survivors, and in severe than in mild cases. CD3+CD127+ lymphocytes were lower in severe than in mild cases; in non-survivors than in survivors and in ICU than in non- ICU admitted cases. sIL7Rα plasmatic levels were higher in non-survivors than in survivors, and in severe than in mild cases. rs6897932CC, rs987106AA and rs3194051GG carriers showed the highest while rs6897932TT showed the lowest sIL7Rα levels. The AUC of sIL7Rα levels predicting 30-day mortality was 0.71. Plasma IL-7 levels were lower in ICU-admitted than in not ICU-admitted and in non-survivors than in survivors. No additional association was detected. In conclusion, rs3194051GG and rs987106TT IL7R genotypes were associated with a poorer prognosis. A significant association between sIL7R levels and SNPs of the IL7R gene is described for the first time in adult CAP. Increased plasmatic sIL7R could contribute to identifying adult CAP cases at risk of death.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Ampuero S, Bahamonde G, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Lizama L, López M, Avendaño LF, Luchsinger V.
- Cómo citar: "Ampuero S, Bahamonde G, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Lizama L, López M, Avendaño LF, Luchsinger V. IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study. Sci Rep.
2022;12(1):13145. doi: 10.1038/s41598-022-13063-x. PMID: 35907923 PMCID: PMC9339003. ISSN: 2045-2322. IF [JCR]: 4.996. Q2"
- Resumen: Community-acquired pneumonia (CAP) is a worldwide leading cause of death. Recognized risk factors in some severe cases have not been identified. Lymphocytopenia has been frequently described in CAP. Since IL-7, membrane-bound receptor (IL7Rα;CD127) and soluble IL7Rα (sIL7R) are critical in lymphocytes homeostasis, in this work we aimed to evaluate the involvement of the IL-7/IL7Rα axis in the severity of adult CAP, since it has not been explored. The IL7Rα SNPs rs6897932, rs987106, and rs3194051 SNPs in IL7α were genotyped, the systemic expression of the IL7R gene, sIL7R, IL-7, and levels of peripheral IL7Rα+ T lymphocytes were quantified in 202 hospitalized CAP cases. rs3194051GG was more frequent in non-survivors than in survivors; rs987106TT was more frequent and rs3194051AA less frequent in patients at intensive care unit (ICU) than in those not admitted to ICU. IL7Rα gene expression was lower in non-survivors than in survivors, and in severe than in mild cases. CD3+CD127+ lymphocytes were lower in severe than in mild cases; in non-survivors than in survivors and in ICU than in non- ICU admitted cases. sIL7Rα plasmatic levels were higher in non-survivors than in survivors, and in severe than in mild cases. rs6897932CC, rs987106AA and rs3194051GG carriers showed the highest while rs6897932TT showed the lowest sIL7Rα levels. The AUC of sIL7Rα levels predicting 30-day mortality was 0.71. Plasma IL-7 levels were lower in ICU-admitted than in not ICU-admitted and in non-survivors than in survivors. No additional association was detected. In conclusion, rs3194051GG and rs987106TT IL7R genotypes were associated with a poorer prognosis. A significant association between sIL7R levels and SNPs of the IL7R gene is described for the first time in adult CAP. Increased plasmatic sIL7R could contribute to identifying adult CAP cases at risk of death.
- Cómo citar: "Ampuero S, Bahamonde G, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Lizama L, López M, Avendaño LF, Luchsinger V. IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study. Sci Rep.
2022;12(1):13145. doi: 10.1038/s41598-022-13063-x. PMID: 35907923 PMCID: PMC9339003. ISSN: 2045-2322. IF [JCR]: 4.996. Q2"
- Resumen: Community-acquired pneumonia (CAP) is a worldwide leading cause of death. Recognized risk factors in some severe cases have not been identified. Lymphocytopenia has been frequently described in CAP. Since IL-7, membrane-bound receptor (IL7Rα;CD127) and soluble IL7Rα (sIL7R) are critical in lymphocytes homeostasis, in this work we aimed to evaluate the involvement of the IL-7/IL7Rα axis in the severity of adult CAP, since it has not been explored. The IL7Rα SNPs rs6897932, rs987106, and rs3194051 SNPs in IL7α were genotyped, the systemic expression of the IL7R gene, sIL7R, IL-7, and levels of peripheral IL7Rα+ T lymphocytes were quantified in 202 hospitalized CAP cases. rs3194051GG was more frequent in non-survivors than in survivors; rs987106TT was more frequent and rs3194051AA less frequent in patients at intensive care unit (ICU) than in those not admitted to ICU. IL7Rα gene expression was lower in non-survivors than in survivors, and in severe than in mild cases. CD3+CD127+ lymphocytes were lower in severe than in mild cases; in non-survivors than in survivors and in ICU than in non- ICU admitted cases. sIL7Rα plasmatic levels were higher in non-survivors than in survivors, and in severe than in mild cases. rs6897932CC, rs987106AA and rs3194051GG carriers showed the highest while rs6897932TT showed the lowest sIL7Rα levels. The AUC of sIL7Rα levels predicting 30-day mortality was 0.71. Plasma IL-7 levels were lower in ICU-admitted than in not ICU-admitted and in non-survivors than in survivors. No additional association was detected. In conclusion, rs3194051GG and rs987106TT IL7R genotypes were associated with a poorer prognosis. A significant association between sIL7R levels and SNPs of the IL7R gene is described for the first time in adult CAP. Increased plasmatic sIL7R could contribute to identifying adult CAP cases at risk of death.
2021
Garmendia, María Luisa
En: Rev. Med. Chile., 2021.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeyb,
title = {Rendimiento de dos índices predictores de mortalidad (PSI y CURB-65) en pacientes adultos inmunocompetentes hospitalizados por neumonía adquirida en la comunidad [Yield of two mortality predictors in immunocompetent patients with community acquired pneumonia].},
author = {María Luisa Garmendia},
doi = {10.4067/S0034-98872021000901275},
year = {2021},
date = {2021-00-00},
urldate = {2021-00-00},
journal = {Rev. Med. Chile.},
abstract = {- Otros autores: Muñoz P, Ruiz M, Pizarro R, Rossi P, Prades Y, Huenchur L, Lizama L, Ampuero S, Larrañaga C, Avedaño LF, Luchsinger V.
- Cómo citar: Muñoz P, Ruiz M, Pizarro R, Rossi P, Prades Y, Huenchur L, Lizama L, Ampuero S, Larrañaga C, Avedaño LF, Luchsinger V. Rendimiento de dos índices predictores de mortalidad (PSI y CURB-65) en pacientes adultos inmunocompetentes hospitalizados por neumonía adquirida en la comunidad [Yield of two mortality predictors in immunocompetent patients with community acquired pneumonia]. Rev. Med. Chile. 2021;149(9):1275-1284. doi: 10.4067/S0034-98872021000901275. PMID: 35319680. ISSN: 0034-9887. IF [JCR] 0.553. Q4
- Resumen: Background: The severity of community acquired pneumonia (CAP) can be evaluated by the PSI and CURB-65 scales. However, it is unknown whether their predictive capacity varies according to the etiology of the disease.
Aim: To compare the performance of these scales in adults with viral, bacterial, mixed, and no agent detected CAP.
Material and Methods: We studied 725 patients hospitalized for CAP aged 18 to 95 years (47% females) Urinary S. pneumoniae and Legionella antigens were detected by immuno-chromatography (Binax®). Respiratory viruses and bacteria were detected by PCR in nasopharyngeal smears. The proportions of deaths, admission to the intensive care unit (ICU), and oxygen therapy were compared between mild and non-severe patients defined by PSI (I/II and I-III) and CURB-65 (1 and 1-2), according to the causative agent. Results: Ten percent of patients died. A causative agent was detected in 65%. The proportion of mild and non-severe patients according to PSI and CURB-65, and of deceased patients, admitted to the ICU and with oxygen therapy was similar in the four categories per agent. There were no deaths among non-severe patients with bacterial CAP. However, 6% of patients with CAP caused by virus or without causative agents, died. No deaths occurred among mild patients with bacterial CAP. In viral CAP, no deaths occurred among patients classified as mild only by PSI. The yields of PSI were greater than those of CURB-65 in non-severe patients who died and were admitted to the ICU with bacterial and viral CAP (5 and 14%; 7 and 12% respectively},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Muñoz P, Ruiz M, Pizarro R, Rossi P, Prades Y, Huenchur L, Lizama L, Ampuero S, Larrañaga C, Avedaño LF, Luchsinger V.
- Cómo citar: Muñoz P, Ruiz M, Pizarro R, Rossi P, Prades Y, Huenchur L, Lizama L, Ampuero S, Larrañaga C, Avedaño LF, Luchsinger V. Rendimiento de dos índices predictores de mortalidad (PSI y CURB-65) en pacientes adultos inmunocompetentes hospitalizados por neumonía adquirida en la comunidad [Yield of two mortality predictors in immunocompetent patients with community acquired pneumonia]. Rev. Med. Chile. 2021;149(9):1275-1284. doi: 10.4067/S0034-98872021000901275. PMID: 35319680. ISSN: 0034-9887. IF [JCR] 0.553. Q4
- Resumen: Background: The severity of community acquired pneumonia (CAP) can be evaluated by the PSI and CURB-65 scales. However, it is unknown whether their predictive capacity varies according to the etiology of the disease.
Aim: To compare the performance of these scales in adults with viral, bacterial, mixed, and no agent detected CAP.
Material and Methods: We studied 725 patients hospitalized for CAP aged 18 to 95 years (47% females) Urinary S. pneumoniae and Legionella antigens were detected by immuno-chromatography (Binax®). Respiratory viruses and bacteria were detected by PCR in nasopharyngeal smears. The proportions of deaths, admission to the intensive care unit (ICU), and oxygen therapy were compared between mild and non-severe patients defined by PSI (I/II and I-III) and CURB-65 (1 and 1-2), according to the causative agent. Results: Ten percent of patients died. A causative agent was detected in 65%. The proportion of mild and non-severe patients according to PSI and CURB-65, and of deceased patients, admitted to the ICU and with oxygen therapy was similar in the four categories per agent. There were no deaths among non-severe patients with bacterial CAP. However, 6% of patients with CAP caused by virus or without causative agents, died. No deaths occurred among mild patients with bacterial CAP. In viral CAP, no deaths occurred among patients classified as mild only by PSI. The yields of PSI were greater than those of CURB-65 in non-severe patients who died and were admitted to the ICU with bacterial and viral CAP (5 and 14%; 7 and 12% respectively
- Cómo citar: Muñoz P, Ruiz M, Pizarro R, Rossi P, Prades Y, Huenchur L, Lizama L, Ampuero S, Larrañaga C, Avedaño LF, Luchsinger V. Rendimiento de dos índices predictores de mortalidad (PSI y CURB-65) en pacientes adultos inmunocompetentes hospitalizados por neumonía adquirida en la comunidad [Yield of two mortality predictors in immunocompetent patients with community acquired pneumonia]. Rev. Med. Chile. 2021;149(9):1275-1284. doi: 10.4067/S0034-98872021000901275. PMID: 35319680. ISSN: 0034-9887. IF [JCR] 0.553. Q4
- Resumen: Background: The severity of community acquired pneumonia (CAP) can be evaluated by the PSI and CURB-65 scales. However, it is unknown whether their predictive capacity varies according to the etiology of the disease.
Aim: To compare the performance of these scales in adults with viral, bacterial, mixed, and no agent detected CAP.
Material and Methods: We studied 725 patients hospitalized for CAP aged 18 to 95 years (47% females) Urinary S. pneumoniae and Legionella antigens were detected by immuno-chromatography (Binax®). Respiratory viruses and bacteria were detected by PCR in nasopharyngeal smears. The proportions of deaths, admission to the intensive care unit (ICU), and oxygen therapy were compared between mild and non-severe patients defined by PSI (I/II and I-III) and CURB-65 (1 and 1-2), according to the causative agent. Results: Ten percent of patients died. A causative agent was detected in 65%. The proportion of mild and non-severe patients according to PSI and CURB-65, and of deceased patients, admitted to the ICU and with oxygen therapy was similar in the four categories per agent. There were no deaths among non-severe patients with bacterial CAP. However, 6% of patients with CAP caused by virus or without causative agents, died. No deaths occurred among mild patients with bacterial CAP. In viral CAP, no deaths occurred among patients classified as mild only by PSI. The yields of PSI were greater than those of CURB-65 in non-severe patients who died and were admitted to the ICU with bacterial and viral CAP (5 and 14%; 7 and 12% respectively
2020
Corvalán, Camila; Garmendia, María Luisa; Ortega, Alejandra
Academically oriented activity Breaks for first-grade chilean students: Development and Pilot testing effectiveness. Artículo de revista
En: Health Educ. Behav., 2020.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeym,
title = {Academically oriented activity Breaks for first-grade chilean students: Development and Pilot testing effectiveness.},
author = {Camila Corvalán and María Luisa Garmendia and Alejandra Ortega},
doi = {10.1177/1090198120912189},
year = {2020},
date = {2020-00-00},
urldate = {2020-00-00},
journal = {Health Educ. Behav.},
abstract = {- Otros autores: Kain J, Marambio P, Rojas J, Muñoz C, Leyton B.
- Cómo citar: Kain J, Ortega A, Marambio P, Rojas J, Muñoz C, Leyton B. Academically oriented activity Breaks for first-grade chilean students: Development and Pilot testing effectiveness. Health Educ. Behav. 2020;47(3):439-448. doi: 10.1177/1090198120912189. PMID: 32188283. ISSN: 1090-1981. IF [JCR]: 2.623. Q2 - Resumen: We developed and pilot tested the effectiveness of a physically active academic program, Active Breaks (AB), whose objective is to increase school time moderate/vigorous physical activity (MVPA) among first graders, through daily 15-minute bouts of MVPA, at the beginning of the first lesson. Initially, 240 cards including one game each were developed and tested in first-grade students from 16 schools in Santiago. Trained observers and school teachers assessed the time, ease, and feasibility of implementation for each card. Barriers and facilitators to implementation were obtained from semistructured interviews to 14 teachers (out of 16). In eight schools (n = 556 students), we compared school time MVPA (with accelerometers) at baseline and follow-up, using test of proportions. One-hundred and twenty cards (games) complied with all aspects. AB were implemented 50% of the time with a duration of 14 minutes (SD = 5). More than 90% of the time, teachers felt competent to conduct AB, and children understood the instructions and enjoyed the activity. The main facilitators included teachers liking physical activity and considering it important, support of principal and school staff, and conducting AB inside the classroom. Barriers included teacher’s workload and having to conduct AB during the first lesson. During the 4-month period of implementation, MVPA increased by 1.5 and 1.2 percentage points in boys and girls, respectively. The set of 120 cards is easy and feasible to implement. Moreover, preliminary results suggest they could be effective in increasing MVPA during school time, although studies with longer follow-ups are needed to assess the validity of these findings.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Kain J, Marambio P, Rojas J, Muñoz C, Leyton B.
- Cómo citar: Kain J, Ortega A, Marambio P, Rojas J, Muñoz C, Leyton B. Academically oriented activity Breaks for first-grade chilean students: Development and Pilot testing effectiveness. Health Educ. Behav. 2020;47(3):439-448. doi: 10.1177/1090198120912189. PMID: 32188283. ISSN: 1090-1981. IF [JCR]: 2.623. Q2 - Resumen: We developed and pilot tested the effectiveness of a physically active academic program, Active Breaks (AB), whose objective is to increase school time moderate/vigorous physical activity (MVPA) among first graders, through daily 15-minute bouts of MVPA, at the beginning of the first lesson. Initially, 240 cards including one game each were developed and tested in first-grade students from 16 schools in Santiago. Trained observers and school teachers assessed the time, ease, and feasibility of implementation for each card. Barriers and facilitators to implementation were obtained from semistructured interviews to 14 teachers (out of 16). In eight schools (n = 556 students), we compared school time MVPA (with accelerometers) at baseline and follow-up, using test of proportions. One-hundred and twenty cards (games) complied with all aspects. AB were implemented 50% of the time with a duration of 14 minutes (SD = 5). More than 90% of the time, teachers felt competent to conduct AB, and children understood the instructions and enjoyed the activity. The main facilitators included teachers liking physical activity and considering it important, support of principal and school staff, and conducting AB inside the classroom. Barriers included teacher’s workload and having to conduct AB during the first lesson. During the 4-month period of implementation, MVPA increased by 1.5 and 1.2 percentage points in boys and girls, respectively. The set of 120 cards is easy and feasible to implement. Moreover, preliminary results suggest they could be effective in increasing MVPA during school time, although studies with longer follow-ups are needed to assess the validity of these findings.
- Cómo citar: Kain J, Ortega A, Marambio P, Rojas J, Muñoz C, Leyton B. Academically oriented activity Breaks for first-grade chilean students: Development and Pilot testing effectiveness. Health Educ. Behav. 2020;47(3):439-448. doi: 10.1177/1090198120912189. PMID: 32188283. ISSN: 1090-1981. IF [JCR]: 2.623. Q2 - Resumen: We developed and pilot tested the effectiveness of a physically active academic program, Active Breaks (AB), whose objective is to increase school time moderate/vigorous physical activity (MVPA) among first graders, through daily 15-minute bouts of MVPA, at the beginning of the first lesson. Initially, 240 cards including one game each were developed and tested in first-grade students from 16 schools in Santiago. Trained observers and school teachers assessed the time, ease, and feasibility of implementation for each card. Barriers and facilitators to implementation were obtained from semistructured interviews to 14 teachers (out of 16). In eight schools (n = 556 students), we compared school time MVPA (with accelerometers) at baseline and follow-up, using test of proportions. One-hundred and twenty cards (games) complied with all aspects. AB were implemented 50% of the time with a duration of 14 minutes (SD = 5). More than 90% of the time, teachers felt competent to conduct AB, and children understood the instructions and enjoyed the activity. The main facilitators included teachers liking physical activity and considering it important, support of principal and school staff, and conducting AB inside the classroom. Barriers included teacher’s workload and having to conduct AB during the first lesson. During the 4-month period of implementation, MVPA increased by 1.5 and 1.2 percentage points in boys and girls, respectively. The set of 120 cards is easy and feasible to implement. Moreover, preliminary results suggest they could be effective in increasing MVPA during school time, although studies with longer follow-ups are needed to assess the validity of these findings.
Corvalán, Camila; Mujica, Fernanda
Nutrition Status in Adult Chilean population: economic, ethnic and sex inequalities in a post-transitional country. Artículo de revista
En: Public Health Nutr., 2020.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_109,
title = {Nutrition Status in Adult Chilean population: economic, ethnic and sex inequalities in a post-transitional country.},
author = {Camila Corvalán and Fernanda Mujica },
doi = {10.1017/S1368980019004439},
year = {2020},
date = {2020-00-00},
urldate = {2020-00-00},
journal = {Public Health Nutr.},
abstract = {- Otros autores: Navarro-Rosenblatt D, López-Arana S.
- Cómo citar: Mujica-Coopman MF, Navarro-Rosenblatt D, López-Arana S, Corvalán C. Nutrition Status in Adult Chilean population: economic, ethnic and sex inequalities in a post-transitional country. Public Health Nutr. 2020;23(S1):1-12. doi: 10.1017/S1368980019004439. PMID: 32131930. ISSN: 1368-9800. IF [JCR]: 4.539. Q2
-Resumen: Objective: To assess the relationship between malnutrition, socioeconomic status (SES) and ethnicity in Chilean adult population.
Design: Nationally representative survey (ENS) conducted in 2016-2017. Sociodemographic information, weight, height and hemoglobin (Hb) were measured (2003 ENS). Excess weight was defined as BMI ≥25 kg/m2. Undernutrition included underweight (BMI <18·5 kg/m2), short stature (height <1·49 m in women and <1·62 m in men) or anaemia (Hb <12 g/l). Education and household income level were used as indicators of SES; ethnicity was self-reported. We applied linear combinations of estimators to compare the prevalence of excess weight and undernutrition by SES and ethnicity.
Setting: Chile.
Participants: In total, 5082 adults ≥20 years (64 % women) and 1739 women ≥20 years for anaemia analyses.
Results: Overall, >75 % of women and men had excess weight. Low SES women either by income or education had higher excess weight ((82·0 (77·1, 86·1) v. 65·0 (54·8, 74·1)) by income; (85·3 (80·6, 89·0) v. 68·2 (61·6, 74·1) %) by education) and short stature (20-49 years; 31(17·9, 48·2) v. 5·2 (2·2,11·4) by education); obesity was also more frequent among indigenous women (20-49 years; 55·8 (44·4, 66·6) v. 37·2 (32·7, 42·0) %) than non-indigenous women. In men, excess weight did not significantly differ by SES or ethnicity, but short stature concentrated in low SES (20-49 years; 47·6 (24·6, 71·6) v. 4·5 (2·1, 9·5) by education) and indigenous men (21·5 (11·9, 5·5, 11·9) v. 8·2 (5·5, 11·9)) (P < 0·05 for all).
Conclusions: In Chile, malnutrition is disproportionately concentrated among women of low SES and indigenous origin; these inequalities should be considered when implementing prevention policies.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Navarro-Rosenblatt D, López-Arana S.
- Cómo citar: Mujica-Coopman MF, Navarro-Rosenblatt D, López-Arana S, Corvalán C. Nutrition Status in Adult Chilean population: economic, ethnic and sex inequalities in a post-transitional country. Public Health Nutr. 2020;23(S1):1-12. doi: 10.1017/S1368980019004439. PMID: 32131930. ISSN: 1368-9800. IF [JCR]: 4.539. Q2
-Resumen: Objective: To assess the relationship between malnutrition, socioeconomic status (SES) and ethnicity in Chilean adult population.
Design: Nationally representative survey (ENS) conducted in 2016-2017. Sociodemographic information, weight, height and hemoglobin (Hb) were measured (2003 ENS). Excess weight was defined as BMI ≥25 kg/m2. Undernutrition included underweight (BMI <18·5 kg/m2), short stature (height <1·49 m in women and <1·62 m in men) or anaemia (Hb <12 g/l). Education and household income level were used as indicators of SES; ethnicity was self-reported. We applied linear combinations of estimators to compare the prevalence of excess weight and undernutrition by SES and ethnicity.
Setting: Chile.
Participants: In total, 5082 adults ≥20 years (64 % women) and 1739 women ≥20 years for anaemia analyses.
Results: Overall, >75 % of women and men had excess weight. Low SES women either by income or education had higher excess weight ((82·0 (77·1, 86·1) v. 65·0 (54·8, 74·1)) by income; (85·3 (80·6, 89·0) v. 68·2 (61·6, 74·1) %) by education) and short stature (20-49 years; 31(17·9, 48·2) v. 5·2 (2·2,11·4) by education); obesity was also more frequent among indigenous women (20-49 years; 55·8 (44·4, 66·6) v. 37·2 (32·7, 42·0) %) than non-indigenous women. In men, excess weight did not significantly differ by SES or ethnicity, but short stature concentrated in low SES (20-49 years; 47·6 (24·6, 71·6) v. 4·5 (2·1, 9·5) by education) and indigenous men (21·5 (11·9, 5·5, 11·9) v. 8·2 (5·5, 11·9)) (P < 0·05 for all).
Conclusions: In Chile, malnutrition is disproportionately concentrated among women of low SES and indigenous origin; these inequalities should be considered when implementing prevention policies.
- Cómo citar: Mujica-Coopman MF, Navarro-Rosenblatt D, López-Arana S, Corvalán C. Nutrition Status in Adult Chilean population: economic, ethnic and sex inequalities in a post-transitional country. Public Health Nutr. 2020;23(S1):1-12. doi: 10.1017/S1368980019004439. PMID: 32131930. ISSN: 1368-9800. IF [JCR]: 4.539. Q2
-Resumen: Objective: To assess the relationship between malnutrition, socioeconomic status (SES) and ethnicity in Chilean adult population.
Design: Nationally representative survey (ENS) conducted in 2016-2017. Sociodemographic information, weight, height and hemoglobin (Hb) were measured (2003 ENS). Excess weight was defined as BMI ≥25 kg/m2. Undernutrition included underweight (BMI <18·5 kg/m2), short stature (height <1·49 m in women and <1·62 m in men) or anaemia (Hb <12 g/l). Education and household income level were used as indicators of SES; ethnicity was self-reported. We applied linear combinations of estimators to compare the prevalence of excess weight and undernutrition by SES and ethnicity.
Setting: Chile.
Participants: In total, 5082 adults ≥20 years (64 % women) and 1739 women ≥20 years for anaemia analyses.
Results: Overall, >75 % of women and men had excess weight. Low SES women either by income or education had higher excess weight ((82·0 (77·1, 86·1) v. 65·0 (54·8, 74·1)) by income; (85·3 (80·6, 89·0) v. 68·2 (61·6, 74·1) %) by education) and short stature (20-49 years; 31(17·9, 48·2) v. 5·2 (2·2,11·4) by education); obesity was also more frequent among indigenous women (20-49 years; 55·8 (44·4, 66·6) v. 37·2 (32·7, 42·0) %) than non-indigenous women. In men, excess weight did not significantly differ by SES or ethnicity, but short stature concentrated in low SES (20-49 years; 47·6 (24·6, 71·6) v. 4·5 (2·1, 9·5) by education) and indigenous men (21·5 (11·9, 5·5, 11·9) v. 8·2 (5·5, 11·9)) (P < 0·05 for all).
Conclusions: In Chile, malnutrition is disproportionately concentrated among women of low SES and indigenous origin; these inequalities should be considered when implementing prevention policies.
Garmendia, María Luisa
Immunoglobulins concentration and B cell counts as severity markers in adult community-acquired pneumonia: Cross sectional study. Artículo de revista
En: Medicine (Baltimore)., 2020.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeyj,
title = {Immunoglobulins concentration and B cell counts as severity markers in adult community-acquired pneumonia: Cross sectional study.},
author = {María Luisa Garmendia},
doi = {10.1097/MD.0000000000022390},
year = {2020},
date = {2020-00-00},
urldate = {2020-00-00},
journal = {Medicine (Baltimore).},
abstract = {- Otros autores: Luchsinger V, Lizama L, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Moreno C, López M, Ampuero S, Larrañaga C, Avendaño LF.
- Cómo citar: Luchsinger V, Lizama L, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Moreno C, López M, Ampuero S, Larrañaga C, Avendaño LF. Immunoglobulins concentration and B cell counts as severity markers in adult community-acquired pneumonia: Cross sectional study. Medicine (Baltimore). 2020;99(45):e22390. doi: 10.1097/MD.0000000000022390. PMID: 33157914. PMCID: PMC7647610 ISSN: 0025-7974. IF [JCR]: 1.889. Q3 - Resumen: Community-acquired pneumonia (CAP) is a worldwide cause of morbidity and mortality. Immunoglobulins (Igs) and B cells quantification studies in CAP are few and show discrepancies. Serum IgA acts as a powerful natural anti-inflammatory factor, but its role in the CAP has not yet been defined. The highly sensitive xMAP Luminex technique allows better immunoglobulins quantification. The aim of this study was to analyze the relation between clinical severity and circulating Igs and B cells in adults with CAP.Igs (M, A, G1, G2, G3, and G4) and B cells were quantified in peripheral blood of 190 Chilean patients ≥18 years old hospitalized for CAP and in 21 adults without respiratory disease, using xMAP Luminex and flow cytometry, respectively. Clinical history was recorded and PSI and CURB-65 scores were calculated for evaluation of clinical severity.The total IgM, IgG2 and total IgG levels were lower in CAP than in asymptomatic adults (P < .05). No significant differences of Igs levels were found between patients classified as severe and mild by PSI and CURB-65 scores. Fatal cases had higher levels of IgA (P < .05). No differences in CD19 B cells frequency was found between CAP and asymptomatic adults (P = .40). In PSI severe cases, CD19 B cells were significantly lower than in mild cases (P = .008). No differences were found in CURB-65 severe and mild groups (P = .11). In fatal cases (11/82) group, CD19 B cells frequency was lower than in 71 survivors (P = .2). No differences in memory B lymphocytes were detected between asymptomatic and CAP adults, severe and mild patients, survivors and fatal cases (P > .05).Serum IgA levels were significantly higher in fatal CAP cases, raising it as a potential biomarker for severe disease considering its relatively universal availability. In PSI severe patients, B cells showed lower levels and could have a role on its physiopathology. Finding new markers rooted in physiopathology could improve the possibility of scoring severe CAP cases. Luminex technology showed promising quantification serum Igs.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Luchsinger V, Lizama L, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Moreno C, López M, Ampuero S, Larrañaga C, Avendaño LF.
- Cómo citar: Luchsinger V, Lizama L, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Moreno C, López M, Ampuero S, Larrañaga C, Avendaño LF. Immunoglobulins concentration and B cell counts as severity markers in adult community-acquired pneumonia: Cross sectional study. Medicine (Baltimore). 2020;99(45):e22390. doi: 10.1097/MD.0000000000022390. PMID: 33157914. PMCID: PMC7647610 ISSN: 0025-7974. IF [JCR]: 1.889. Q3 - Resumen: Community-acquired pneumonia (CAP) is a worldwide cause of morbidity and mortality. Immunoglobulins (Igs) and B cells quantification studies in CAP are few and show discrepancies. Serum IgA acts as a powerful natural anti-inflammatory factor, but its role in the CAP has not yet been defined. The highly sensitive xMAP Luminex technique allows better immunoglobulins quantification. The aim of this study was to analyze the relation between clinical severity and circulating Igs and B cells in adults with CAP.Igs (M, A, G1, G2, G3, and G4) and B cells were quantified in peripheral blood of 190 Chilean patients ≥18 years old hospitalized for CAP and in 21 adults without respiratory disease, using xMAP Luminex and flow cytometry, respectively. Clinical history was recorded and PSI and CURB-65 scores were calculated for evaluation of clinical severity.The total IgM, IgG2 and total IgG levels were lower in CAP than in asymptomatic adults (P < .05). No significant differences of Igs levels were found between patients classified as severe and mild by PSI and CURB-65 scores. Fatal cases had higher levels of IgA (P < .05). No differences in CD19 B cells frequency was found between CAP and asymptomatic adults (P = .40). In PSI severe cases, CD19 B cells were significantly lower than in mild cases (P = .008). No differences were found in CURB-65 severe and mild groups (P = .11). In fatal cases (11/82) group, CD19 B cells frequency was lower than in 71 survivors (P = .2). No differences in memory B lymphocytes were detected between asymptomatic and CAP adults, severe and mild patients, survivors and fatal cases (P > .05).Serum IgA levels were significantly higher in fatal CAP cases, raising it as a potential biomarker for severe disease considering its relatively universal availability. In PSI severe patients, B cells showed lower levels and could have a role on its physiopathology. Finding new markers rooted in physiopathology could improve the possibility of scoring severe CAP cases. Luminex technology showed promising quantification serum Igs.
- Cómo citar: Luchsinger V, Lizama L, Tempio F, Ruiz M, Pizarro R, Rossi P, Huenchur L, Moreno C, López M, Ampuero S, Larrañaga C, Avendaño LF. Immunoglobulins concentration and B cell counts as severity markers in adult community-acquired pneumonia: Cross sectional study. Medicine (Baltimore). 2020;99(45):e22390. doi: 10.1097/MD.0000000000022390. PMID: 33157914. PMCID: PMC7647610 ISSN: 0025-7974. IF [JCR]: 1.889. Q3 - Resumen: Community-acquired pneumonia (CAP) is a worldwide cause of morbidity and mortality. Immunoglobulins (Igs) and B cells quantification studies in CAP are few and show discrepancies. Serum IgA acts as a powerful natural anti-inflammatory factor, but its role in the CAP has not yet been defined. The highly sensitive xMAP Luminex technique allows better immunoglobulins quantification. The aim of this study was to analyze the relation between clinical severity and circulating Igs and B cells in adults with CAP.Igs (M, A, G1, G2, G3, and G4) and B cells were quantified in peripheral blood of 190 Chilean patients ≥18 years old hospitalized for CAP and in 21 adults without respiratory disease, using xMAP Luminex and flow cytometry, respectively. Clinical history was recorded and PSI and CURB-65 scores were calculated for evaluation of clinical severity.The total IgM, IgG2 and total IgG levels were lower in CAP than in asymptomatic adults (P < .05). No significant differences of Igs levels were found between patients classified as severe and mild by PSI and CURB-65 scores. Fatal cases had higher levels of IgA (P < .05). No differences in CD19 B cells frequency was found between CAP and asymptomatic adults (P = .40). In PSI severe cases, CD19 B cells were significantly lower than in mild cases (P = .008). No differences were found in CURB-65 severe and mild groups (P = .11). In fatal cases (11/82) group, CD19 B cells frequency was lower than in 71 survivors (P = .2). No differences in memory B lymphocytes were detected between asymptomatic and CAP adults, severe and mild patients, survivors and fatal cases (P > .05).Serum IgA levels were significantly higher in fatal CAP cases, raising it as a potential biomarker for severe disease considering its relatively universal availability. In PSI severe patients, B cells showed lower levels and could have a role on its physiopathology. Finding new markers rooted in physiopathology could improve the possibility of scoring severe CAP cases. Luminex technology showed promising quantification serum Igs.
2017
Corvalán, Camila
Reply to AT Wijayabahu. Artículo de revista
En: Am. J. Clin. Nutr., 2017.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_166,
title = {Reply to AT Wijayabahu.},
author = {Camila Corvalán},
doi = {10.3945/ajcn.117.160176},
year = {2017},
date = {2017-00-00},
urldate = {2017-00-00},
journal = {Am. J. Clin. Nutr.},
abstract = {- Otros autores: Gaskins JA, Michels KB.
- Cómo citar: Gaskins JA, Michels KB. Reply to AT Wijayabahu. Am. J. Clin. Nutr. 2017;106(2):707. doi: 10.3945/ajcn.117.160176 N/A. ISSN: 0002-9165. IF [JCR]: 8.475. Q1},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Gaskins JA, Michels KB.
- Cómo citar: Gaskins JA, Michels KB. Reply to AT Wijayabahu. Am. J. Clin. Nutr. 2017;106(2):707. doi: 10.3945/ajcn.117.160176 N/A. ISSN: 0002-9165. IF [JCR]: 8.475. Q1
- Cómo citar: Gaskins JA, Michels KB. Reply to AT Wijayabahu. Am. J. Clin. Nutr. 2017;106(2):707. doi: 10.3945/ajcn.117.160176 N/A. ISSN: 0002-9165. IF [JCR]: 8.475. Q1
2016
Garmendia, María Luisa
Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia. Artículo de revista
En: J. Med. Virol., 2016.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeyx,
title = {Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia.},
author = {María Luisa Garmendia},
doi = {10.1002/jmv.24463},
year = {2016},
date = {2016-00-00},
urldate = {2016-00-00},
journal = {J. Med. Virol.},
abstract = {- Otros autores: Luchsinger V, Prades Y, Ruiz M, Pizarro R, Rossi P, Lizama L, Meza A, Larrañaga C, Avendaño LF.
- Cómo citar: Luchsinger V, Prades Y, Ruiz M, Pizarro R, Rossi P, Lizama L, Meza A, Larrañaga C, Avendaño LF. Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia. J. Med. Virol. 2016;88(7):1173-1179. doi: 10.1002/jmv.24463. PMID: 27061405. ISSN: 0146-6615. IF [JCR]: 2.327. Q4 - Resumen: Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) (P < 0.0). The sensitivity, specificity, and kappa coefficient of xTAG® RVP compared with rtRT-PCR were 84.2%, 79.6%, and 0.62%, respectively. Luminex® and rtRT-PCR detected 65 (58.0%) and 57 (50.9%) viruses in 112 NPA and 35 (34.3%) and 31 (30.4%) in 102 NPS, respectively (P < 0.01). xTAG® RVP is appropriate for detecting respiratory viruses in CAP adults. Both molecular techniques yielded better results with nasopharyngeal aspirate than swabs.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Luchsinger V, Prades Y, Ruiz M, Pizarro R, Rossi P, Lizama L, Meza A, Larrañaga C, Avendaño LF.
- Cómo citar: Luchsinger V, Prades Y, Ruiz M, Pizarro R, Rossi P, Lizama L, Meza A, Larrañaga C, Avendaño LF. Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia. J. Med. Virol. 2016;88(7):1173-1179. doi: 10.1002/jmv.24463. PMID: 27061405. ISSN: 0146-6615. IF [JCR]: 2.327. Q4 - Resumen: Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) (P < 0.0). The sensitivity, specificity, and kappa coefficient of xTAG® RVP compared with rtRT-PCR were 84.2%, 79.6%, and 0.62%, respectively. Luminex® and rtRT-PCR detected 65 (58.0%) and 57 (50.9%) viruses in 112 NPA and 35 (34.3%) and 31 (30.4%) in 102 NPS, respectively (P < 0.01). xTAG® RVP is appropriate for detecting respiratory viruses in CAP adults. Both molecular techniques yielded better results with nasopharyngeal aspirate than swabs.
- Cómo citar: Luchsinger V, Prades Y, Ruiz M, Pizarro R, Rossi P, Lizama L, Meza A, Larrañaga C, Avendaño LF. Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia. J. Med. Virol. 2016;88(7):1173-1179. doi: 10.1002/jmv.24463. PMID: 27061405. ISSN: 0146-6615. IF [JCR]: 2.327. Q4 - Resumen: Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) (P < 0.0). The sensitivity, specificity, and kappa coefficient of xTAG® RVP compared with rtRT-PCR were 84.2%, 79.6%, and 0.62%, respectively. Luminex® and rtRT-PCR detected 65 (58.0%) and 57 (50.9%) viruses in 112 NPA and 35 (34.3%) and 31 (30.4%) in 102 NPS, respectively (P < 0.01). xTAG® RVP is appropriate for detecting respiratory viruses in CAP adults. Both molecular techniques yielded better results with nasopharyngeal aspirate than swabs.
Garmendia, María Luisa
Estimated glomerular filtration rate, urine albumin excretion, and survival among patients consulting in public Chilean public primary care clinics. Artículo de revista
En: Ren. Fail., 2016.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeyw,
title = {Estimated glomerular filtration rate, urine albumin excretion, and survival among patients consulting in public Chilean public primary care clinics.},
author = {María Luisa Garmendia},
doi = {10.3109/0886022X.2015.1136892},
year = {2016},
date = {2016-00-00},
urldate = {2016-00-00},
journal = {Ren. Fail.},
abstract = {- Otros autores: Rios A, Lorca E, Hirsch S, Sandoval V, Bunout D.
- Cómo citar: Rios A, Lorca E, Hirsch S, Sandoval V, Bunout D. Estimated glomerular filtration rate, urine albumin excretion, and survival among patients consulting in public Chilean public primary care clinics. Ren. Fail. 2016;38(3):397-403. doi: 10.3109/0886022X.2015.1136892. PMID: 26765359. ISSN: 0886-022X. IF [JCR]: 2.606. Q3
- Resumen: Chronic renal disease (CRD) in its pre-dialysis stage is an important risk factor for mortality among adults. The aim of this study was to assess the effects of CRD on mortality among consultants in Chilean public primary care clinics. We obtained information about serum creatinine, urinary albumin excretion (UAE), blood pressure, and body mass index of 5224 consultants [3379 females aged 67 (59-75) years and 1845 males aged 68 (59-75) years] in three clinics of Metropolitan Santiago. Kaplan-Meier curves and Cox proportional hazard regression models were used to determine risk factors for mortality, determined 41 months after obtaining the blood samples. During the follow-up period, 262 patients died (33% due to circulatory causes and 29% due to tumors). Kaplan-Meier curves showed that there was a significant association between survival, estimated glomerular filtration rate, and UAE. Cox models showed that serum creatinine, UAE, a lower body mass index, and a history of diabetes were significant mortality predictors. A sensitivity analysis performed eliminating extreme ages (less than 50 and more than 80 years), included high diastolic pressure as a predictor of survival. We conclude that among patients with CRD in its pre-dialysis stage, UAE is an important predictor of survival, along with serum creatinine. A low body mass index was associated with a higher mortality.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Rios A, Lorca E, Hirsch S, Sandoval V, Bunout D.
- Cómo citar: Rios A, Lorca E, Hirsch S, Sandoval V, Bunout D. Estimated glomerular filtration rate, urine albumin excretion, and survival among patients consulting in public Chilean public primary care clinics. Ren. Fail. 2016;38(3):397-403. doi: 10.3109/0886022X.2015.1136892. PMID: 26765359. ISSN: 0886-022X. IF [JCR]: 2.606. Q3
- Resumen: Chronic renal disease (CRD) in its pre-dialysis stage is an important risk factor for mortality among adults. The aim of this study was to assess the effects of CRD on mortality among consultants in Chilean public primary care clinics. We obtained information about serum creatinine, urinary albumin excretion (UAE), blood pressure, and body mass index of 5224 consultants [3379 females aged 67 (59-75) years and 1845 males aged 68 (59-75) years] in three clinics of Metropolitan Santiago. Kaplan-Meier curves and Cox proportional hazard regression models were used to determine risk factors for mortality, determined 41 months after obtaining the blood samples. During the follow-up period, 262 patients died (33% due to circulatory causes and 29% due to tumors). Kaplan-Meier curves showed that there was a significant association between survival, estimated glomerular filtration rate, and UAE. Cox models showed that serum creatinine, UAE, a lower body mass index, and a history of diabetes were significant mortality predictors. A sensitivity analysis performed eliminating extreme ages (less than 50 and more than 80 years), included high diastolic pressure as a predictor of survival. We conclude that among patients with CRD in its pre-dialysis stage, UAE is an important predictor of survival, along with serum creatinine. A low body mass index was associated with a higher mortality.
- Cómo citar: Rios A, Lorca E, Hirsch S, Sandoval V, Bunout D. Estimated glomerular filtration rate, urine albumin excretion, and survival among patients consulting in public Chilean public primary care clinics. Ren. Fail. 2016;38(3):397-403. doi: 10.3109/0886022X.2015.1136892. PMID: 26765359. ISSN: 0886-022X. IF [JCR]: 2.606. Q3
- Resumen: Chronic renal disease (CRD) in its pre-dialysis stage is an important risk factor for mortality among adults. The aim of this study was to assess the effects of CRD on mortality among consultants in Chilean public primary care clinics. We obtained information about serum creatinine, urinary albumin excretion (UAE), blood pressure, and body mass index of 5224 consultants [3379 females aged 67 (59-75) years and 1845 males aged 68 (59-75) years] in three clinics of Metropolitan Santiago. Kaplan-Meier curves and Cox proportional hazard regression models were used to determine risk factors for mortality, determined 41 months after obtaining the blood samples. During the follow-up period, 262 patients died (33% due to circulatory causes and 29% due to tumors). Kaplan-Meier curves showed that there was a significant association between survival, estimated glomerular filtration rate, and UAE. Cox models showed that serum creatinine, UAE, a lower body mass index, and a history of diabetes were significant mortality predictors. A sensitivity analysis performed eliminating extreme ages (less than 50 and more than 80 years), included high diastolic pressure as a predictor of survival. We conclude that among patients with CRD in its pre-dialysis stage, UAE is an important predictor of survival, along with serum creatinine. A low body mass index was associated with a higher mortality.
2015
Garmendia, María Luisa
En: Rev. Chil. Infectol., 2015.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokeyy,
title = {Antigenemia y reacción de polimerasa en cadena en tiempo real en el diagnóstico de enfermedad por citomegalovirus en adultos con virus de inmunodeficiencia adquirida [Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults].},
author = {María Luisa Garmendia},
doi = {10.4067/S0716-10182015000700008},
year = {2015},
date = {2015-00-00},
urldate = {2015-00-00},
journal = {Rev. Chil. Infectol.},
abstract = {- Otros autores: Luchsinger V, Vásquez P, Silva M, Bruno MJ, Siches I, Villarroel J, Larrañaga C.
- Cómo citar: Luchsinger V, Vásquez P, Silva M, Bruno MJ, Siches I, Villarroel J, Larrañaga C. Antigenemia y reacción de polimerasa en cadena en tiempo real en el diagnóstico de enfermedad por citomegalovirus en adultos con virus de inmunodeficiencia adquirida [Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults]. Rev. Chil. Infectol. 2015;32(6):664-71. doi: 10.4067/S0716-10182015000700008. PMID: 26928503. ISSN: 0716-1018. IF [JCR]: 0.520. Q4
- Resumen: La infección por citomegalovirus (CMV) es frecuente en adultos con virus de inmunodeficiencia humana (VIH). No se ha establecido la utilidad de los métodos cuantitativos para diagnosticar enfermedad por CMV en pacientes chilenos. Objetivo: Determinar la positividad de antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) en el diagnóstico de enfermedad por CMV en adultos chilenos con infección por VIH. Metodología: Se detectó CMV mediante aislamiento viral rápido (AVR), antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) cuantitativa en adultos infectados por VIH, con y sin sospecha de enfermedad por CMV. Resultados: El recuento de LT CD4 fue menor y mayor la carga de VIH en 102 sintomáticos respecto a 77 asintomáticos (p < 0,05). La antigenemia y la RPC-TR fueron positivas en 46 y 36% de los enfermos y en 3 y 5% de los asintomáticos respectivamente. La sensibilidad, especificidad, valor predictor positivo y negativo de la antigenemia y la RPC-TR fueron 92%, 80%, 72% y 95% y 72%, 95%, 92% y 80%, respectivamente. Los valores de corte fueron ≥ 1 núcleo (+) y ≥ 5,5 log10 copias/2 x 106 céls. Se aisló CMV en 3,4%, todos los sintomáticos. Conclusión: La antigenemia y la RPC-TR tienen una positividad similar para diagnosticar enfermedad por CMV en adultos chilenos con infección por VIH. El AVR es inapropiado como referencia por su baja positividad.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Luchsinger V, Vásquez P, Silva M, Bruno MJ, Siches I, Villarroel J, Larrañaga C.
- Cómo citar: Luchsinger V, Vásquez P, Silva M, Bruno MJ, Siches I, Villarroel J, Larrañaga C. Antigenemia y reacción de polimerasa en cadena en tiempo real en el diagnóstico de enfermedad por citomegalovirus en adultos con virus de inmunodeficiencia adquirida [Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults]. Rev. Chil. Infectol. 2015;32(6):664-71. doi: 10.4067/S0716-10182015000700008. PMID: 26928503. ISSN: 0716-1018. IF [JCR]: 0.520. Q4
- Resumen: La infección por citomegalovirus (CMV) es frecuente en adultos con virus de inmunodeficiencia humana (VIH). No se ha establecido la utilidad de los métodos cuantitativos para diagnosticar enfermedad por CMV en pacientes chilenos. Objetivo: Determinar la positividad de antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) en el diagnóstico de enfermedad por CMV en adultos chilenos con infección por VIH. Metodología: Se detectó CMV mediante aislamiento viral rápido (AVR), antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) cuantitativa en adultos infectados por VIH, con y sin sospecha de enfermedad por CMV. Resultados: El recuento de LT CD4 fue menor y mayor la carga de VIH en 102 sintomáticos respecto a 77 asintomáticos (p < 0,05). La antigenemia y la RPC-TR fueron positivas en 46 y 36% de los enfermos y en 3 y 5% de los asintomáticos respectivamente. La sensibilidad, especificidad, valor predictor positivo y negativo de la antigenemia y la RPC-TR fueron 92%, 80%, 72% y 95% y 72%, 95%, 92% y 80%, respectivamente. Los valores de corte fueron ≥ 1 núcleo (+) y ≥ 5,5 log10 copias/2 x 106 céls. Se aisló CMV en 3,4%, todos los sintomáticos. Conclusión: La antigenemia y la RPC-TR tienen una positividad similar para diagnosticar enfermedad por CMV en adultos chilenos con infección por VIH. El AVR es inapropiado como referencia por su baja positividad.
- Cómo citar: Luchsinger V, Vásquez P, Silva M, Bruno MJ, Siches I, Villarroel J, Larrañaga C. Antigenemia y reacción de polimerasa en cadena en tiempo real en el diagnóstico de enfermedad por citomegalovirus en adultos con virus de inmunodeficiencia adquirida [Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults]. Rev. Chil. Infectol. 2015;32(6):664-71. doi: 10.4067/S0716-10182015000700008. PMID: 26928503. ISSN: 0716-1018. IF [JCR]: 0.520. Q4
- Resumen: La infección por citomegalovirus (CMV) es frecuente en adultos con virus de inmunodeficiencia humana (VIH). No se ha establecido la utilidad de los métodos cuantitativos para diagnosticar enfermedad por CMV en pacientes chilenos. Objetivo: Determinar la positividad de antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) en el diagnóstico de enfermedad por CMV en adultos chilenos con infección por VIH. Metodología: Se detectó CMV mediante aislamiento viral rápido (AVR), antigenemia y reacción de polimerasa en cadena en tiempo real (RPC-TR) cuantitativa en adultos infectados por VIH, con y sin sospecha de enfermedad por CMV. Resultados: El recuento de LT CD4 fue menor y mayor la carga de VIH en 102 sintomáticos respecto a 77 asintomáticos (p < 0,05). La antigenemia y la RPC-TR fueron positivas en 46 y 36% de los enfermos y en 3 y 5% de los asintomáticos respectivamente. La sensibilidad, especificidad, valor predictor positivo y negativo de la antigenemia y la RPC-TR fueron 92%, 80%, 72% y 95% y 72%, 95%, 92% y 80%, respectivamente. Los valores de corte fueron ≥ 1 núcleo (+) y ≥ 5,5 log10 copias/2 x 106 céls. Se aisló CMV en 3,4%, todos los sintomáticos. Conclusión: La antigenemia y la RPC-TR tienen una positividad similar para diagnosticar enfermedad por CMV en adultos chilenos con infección por VIH. El AVR es inapropiado como referencia por su baja positividad.
Reyes, Marcela
Preadipocyte proliferation is elevated by calcium sensing receptor activation. Artículo de revista
En: Mol. Cell. Endocrinol., 2015.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_185,
title = {Preadipocyte proliferation is elevated by calcium sensing receptor activation.},
author = {Marcela Reyes},
doi = {10.1016/j.mce.2015.05.011},
year = {2015},
date = {2015-00-00},
urldate = {2015-00-00},
journal = {Mol. Cell. Endocrinol.},
abstract = {- Otros autores: Rocha G, Villalobos E, Fuentes C, Villarroel P, Díaz X, Mattar P, Cifuentes M.
- Cómo citar: Rocha G, Villalobos E, Fuentes C, Villarroel P, Díaz X, Mattar P, Cifuentes M. Preadipocyte proliferation is elevated by calcium sensing receptor activation. Mol. Cell. Endocrinol. 2015;412:251-6. doi: 10.1016/j.mce.2015.05.011. PMID: 25986659. ISSN: 0303-7207. IF [JCR]: 4.102. Q2
-Resumen: Obesity is a major worldwide problem, despite considerable efforts against it. While excess body fat defines obesity, adipose tissue quality and functionality are key to whether cardiovascular and metabolic comorbidities develop. Adipose tissue cellular composition can vary considerably, and excess adipocyte progenitors (preadipocytes) is associated with obesity. We have proposed that calcium sensing receptor (CaSR) activation in adipose tissue leads to dysfunction. This study evaluated whether CaSR activation elevates preadipocyte proliferation. Human LS14 preadipocytes were exposed to CaSR activators cinacalcet (2 µM), GdCl3 (5 µM) and spermine (1 µM), and cell viability was evaluated after 72h. CaSR activators elevated proliferation by 19-24%, and CaSR silencing (siRNA) abolished the effect. Cinacalcet elevated phospho-ERK1/2 content, and upstream inhibition of ERK1/2 phosphorylation reverted cinacalcet-induced proliferation. Cinacalcet also elevated expression of the proinflammatory factors IL1β, IL6 and CCL2. The results suggest that CaSR induces preadipocyte proliferation, partly through ERK1/2 activation. Considering reported proinflammatory and adipogenic CaSR effects, excess preadipocyte proliferation further supports the dysfunctional effect of CaSR in obesity.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Rocha G, Villalobos E, Fuentes C, Villarroel P, Díaz X, Mattar P, Cifuentes M.
- Cómo citar: Rocha G, Villalobos E, Fuentes C, Villarroel P, Díaz X, Mattar P, Cifuentes M. Preadipocyte proliferation is elevated by calcium sensing receptor activation. Mol. Cell. Endocrinol. 2015;412:251-6. doi: 10.1016/j.mce.2015.05.011. PMID: 25986659. ISSN: 0303-7207. IF [JCR]: 4.102. Q2
-Resumen: Obesity is a major worldwide problem, despite considerable efforts against it. While excess body fat defines obesity, adipose tissue quality and functionality are key to whether cardiovascular and metabolic comorbidities develop. Adipose tissue cellular composition can vary considerably, and excess adipocyte progenitors (preadipocytes) is associated with obesity. We have proposed that calcium sensing receptor (CaSR) activation in adipose tissue leads to dysfunction. This study evaluated whether CaSR activation elevates preadipocyte proliferation. Human LS14 preadipocytes were exposed to CaSR activators cinacalcet (2 µM), GdCl3 (5 µM) and spermine (1 µM), and cell viability was evaluated after 72h. CaSR activators elevated proliferation by 19-24%, and CaSR silencing (siRNA) abolished the effect. Cinacalcet elevated phospho-ERK1/2 content, and upstream inhibition of ERK1/2 phosphorylation reverted cinacalcet-induced proliferation. Cinacalcet also elevated expression of the proinflammatory factors IL1β, IL6 and CCL2. The results suggest that CaSR induces preadipocyte proliferation, partly through ERK1/2 activation. Considering reported proinflammatory and adipogenic CaSR effects, excess preadipocyte proliferation further supports the dysfunctional effect of CaSR in obesity.
- Cómo citar: Rocha G, Villalobos E, Fuentes C, Villarroel P, Díaz X, Mattar P, Cifuentes M. Preadipocyte proliferation is elevated by calcium sensing receptor activation. Mol. Cell. Endocrinol. 2015;412:251-6. doi: 10.1016/j.mce.2015.05.011. PMID: 25986659. ISSN: 0303-7207. IF [JCR]: 4.102. Q2
-Resumen: Obesity is a major worldwide problem, despite considerable efforts against it. While excess body fat defines obesity, adipose tissue quality and functionality are key to whether cardiovascular and metabolic comorbidities develop. Adipose tissue cellular composition can vary considerably, and excess adipocyte progenitors (preadipocytes) is associated with obesity. We have proposed that calcium sensing receptor (CaSR) activation in adipose tissue leads to dysfunction. This study evaluated whether CaSR activation elevates preadipocyte proliferation. Human LS14 preadipocytes were exposed to CaSR activators cinacalcet (2 µM), GdCl3 (5 µM) and spermine (1 µM), and cell viability was evaluated after 72h. CaSR activators elevated proliferation by 19-24%, and CaSR silencing (siRNA) abolished the effect. Cinacalcet elevated phospho-ERK1/2 content, and upstream inhibition of ERK1/2 phosphorylation reverted cinacalcet-induced proliferation. Cinacalcet also elevated expression of the proinflammatory factors IL1β, IL6 and CCL2. The results suggest that CaSR induces preadipocyte proliferation, partly through ERK1/2 activation. Considering reported proinflammatory and adipogenic CaSR effects, excess preadipocyte proliferation further supports the dysfunctional effect of CaSR in obesity.
2014
Reyes, Marcela
Calcium, obesity, and the role of the calcium-sensing receptor. Artículo de revista
En: Nutr. Rev., 2014.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_191,
title = {Calcium, obesity, and the role of the calcium-sensing receptor.},
author = {Marcela Reyes},
doi = {10.1111/nure.12135},
year = {2014},
date = {2014-00-00},
urldate = {2014-00-00},
journal = {Nutr. Rev.},
abstract = {- Otros autores: Villarroel P, Villalobos E, Cifuentes M.
- Cómo citar: Villarroel P, Villalobos E, Cifuentes M. Calcium, obesity, and the role of the calcium-sensing receptor. Nutr. Rev. 2014;72(10):627-37. doi: 10.1111/nure.12135. PMID: 25182976. ISSN: 0029-6643. IF [JCR]: 6.846. Q1
-Resumen: The elevated prevalence of obesity worldwide is a challenging public health problem. Dietary calcium intake is frequently below recommendations, and evidence gathered for more than a decade suggests that inadequate calcium intake may be related to increased body weight and/or body fat, although a consensus has yet to be reached. Whole-body energy balance and the cellular mechanisms involved have been proposed to explain this relationship, and increasing evidence from epidemiological, clinical, and basic research lends support to the hypothesis that calcium is linked to the regulation of body weight. This review provides a critical appraisal of evidence from studies that examined several different aspects of this issue. Different mechanisms are highlighted and, based on recent work, new perspectives are offered, which incorporate the concept of obesity-associated inflammation and the possible role of the extracellular calcium-sensing receptor.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Villarroel P, Villalobos E, Cifuentes M.
- Cómo citar: Villarroel P, Villalobos E, Cifuentes M. Calcium, obesity, and the role of the calcium-sensing receptor. Nutr. Rev. 2014;72(10):627-37. doi: 10.1111/nure.12135. PMID: 25182976. ISSN: 0029-6643. IF [JCR]: 6.846. Q1
-Resumen: The elevated prevalence of obesity worldwide is a challenging public health problem. Dietary calcium intake is frequently below recommendations, and evidence gathered for more than a decade suggests that inadequate calcium intake may be related to increased body weight and/or body fat, although a consensus has yet to be reached. Whole-body energy balance and the cellular mechanisms involved have been proposed to explain this relationship, and increasing evidence from epidemiological, clinical, and basic research lends support to the hypothesis that calcium is linked to the regulation of body weight. This review provides a critical appraisal of evidence from studies that examined several different aspects of this issue. Different mechanisms are highlighted and, based on recent work, new perspectives are offered, which incorporate the concept of obesity-associated inflammation and the possible role of the extracellular calcium-sensing receptor.
- Cómo citar: Villarroel P, Villalobos E, Cifuentes M. Calcium, obesity, and the role of the calcium-sensing receptor. Nutr. Rev. 2014;72(10):627-37. doi: 10.1111/nure.12135. PMID: 25182976. ISSN: 0029-6643. IF [JCR]: 6.846. Q1
-Resumen: The elevated prevalence of obesity worldwide is a challenging public health problem. Dietary calcium intake is frequently below recommendations, and evidence gathered for more than a decade suggests that inadequate calcium intake may be related to increased body weight and/or body fat, although a consensus has yet to be reached. Whole-body energy balance and the cellular mechanisms involved have been proposed to explain this relationship, and increasing evidence from epidemiological, clinical, and basic research lends support to the hypothesis that calcium is linked to the regulation of body weight. This review provides a critical appraisal of evidence from studies that examined several different aspects of this issue. Different mechanisms are highlighted and, based on recent work, new perspectives are offered, which incorporate the concept of obesity-associated inflammation and the possible role of the extracellular calcium-sensing receptor.
2013
Garmendia, María Luisa
Adherence to a physical activity intervention among older adults in a post-transitional middle income country: A quantitative and qualitative analysis. Artículo de revista
En: J. Nutr. Health Aging., 2013.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_204,
title = {Adherence to a physical activity intervention among older adults in a post-transitional middle income country: A quantitative and qualitative analysis.},
author = {María Luisa Garmendia},
doi = {10.1007/s12603-012-0417-1},
year = {2013},
date = {2013-00-00},
urldate = {2013-00-00},
journal = {J. Nutr. Health Aging.},
abstract = {- Otros autores: Dangour AD, Albala C, Eguiguren P, Allen E, Uauy R.
- Cómo citar: Dangour AD, Albala C, Eguiguren P, Allen E, Uauy R. Adherence to a physical activity intervention among older adults in a post-transitional middle income country: A quantitative and qualitative analysis. J. Nutr. Health Aging. 2013;17(5):466-471. doi: 10.1007/s12603-012-0417-1. PMID: 23636549 / PMCID: PMC5388178. ISSN: 1279-7707. IF [JCR]: 5.285. Q2
-Resumen: Objectives: The effectiveness of community level interventions depends to a great extent on adherence. Currently, information on factors related to adherence in older adults from developing countries is scarce. Our aim was to identify factors associated to adherence to a physical activity intervention in older adults from a post-transitional middle income country.
Design, setting and participants: Using a combination of quantitative and qualitative methods we studied 996 older Chilean subjects (65-67.9 years at baseline) with low to medium socioeconomic status from 10 health centers randomized to receive a physical activity intervention as part of the CENEX cluster trial (ISRCTN48153354). Measurements: Using a multilevel regression model, the relationship between adherence (defined a priori as attendance at a minimum of 24 physical activity classes spread over at least 12 months) and individual, intervention-related and contextual factors was evaluated. We also conducted 40 semi-structured interviews with older adults (n=36) and instructors (n=4). Transcripts of the interviews were analyzed using content analysis to identify barriers and facilitators to adherence.
Results: Adherence to physical activity intervention was 42.6% (CI 95% 39.5 to 45.6). Depression, diabetes mellitus, percentage of impoverished households and rate of arrests for violent crimes in the neighborhood predicted less adherence (p<0.05) while being retired, participation in physical activity prior to the intervention, and green areas per habitant were positively associated with adherence (p<0.05). The qualitative interviews identified three primary barriers to adherence: current health problems, lack of time due to commitments for caring for family members, and being employed, and two primary facilitators to adherence: the health benefits attributed to the intervention and the opportunity the classes provided for social interaction with others.
Conclusion: In order to enhance effectiveness of community exercise interventions, strategies to improve participation should be targeted to older adults from deprived areas and those with psychological and medical conditions.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Dangour AD, Albala C, Eguiguren P, Allen E, Uauy R.
- Cómo citar: Dangour AD, Albala C, Eguiguren P, Allen E, Uauy R. Adherence to a physical activity intervention among older adults in a post-transitional middle income country: A quantitative and qualitative analysis. J. Nutr. Health Aging. 2013;17(5):466-471. doi: 10.1007/s12603-012-0417-1. PMID: 23636549 / PMCID: PMC5388178. ISSN: 1279-7707. IF [JCR]: 5.285. Q2
-Resumen: Objectives: The effectiveness of community level interventions depends to a great extent on adherence. Currently, information on factors related to adherence in older adults from developing countries is scarce. Our aim was to identify factors associated to adherence to a physical activity intervention in older adults from a post-transitional middle income country.
Design, setting and participants: Using a combination of quantitative and qualitative methods we studied 996 older Chilean subjects (65-67.9 years at baseline) with low to medium socioeconomic status from 10 health centers randomized to receive a physical activity intervention as part of the CENEX cluster trial (ISRCTN48153354). Measurements: Using a multilevel regression model, the relationship between adherence (defined a priori as attendance at a minimum of 24 physical activity classes spread over at least 12 months) and individual, intervention-related and contextual factors was evaluated. We also conducted 40 semi-structured interviews with older adults (n=36) and instructors (n=4). Transcripts of the interviews were analyzed using content analysis to identify barriers and facilitators to adherence.
Results: Adherence to physical activity intervention was 42.6% (CI 95% 39.5 to 45.6). Depression, diabetes mellitus, percentage of impoverished households and rate of arrests for violent crimes in the neighborhood predicted less adherence (p<0.05) while being retired, participation in physical activity prior to the intervention, and green areas per habitant were positively associated with adherence (p<0.05). The qualitative interviews identified three primary barriers to adherence: current health problems, lack of time due to commitments for caring for family members, and being employed, and two primary facilitators to adherence: the health benefits attributed to the intervention and the opportunity the classes provided for social interaction with others.
Conclusion: In order to enhance effectiveness of community exercise interventions, strategies to improve participation should be targeted to older adults from deprived areas and those with psychological and medical conditions.
- Cómo citar: Dangour AD, Albala C, Eguiguren P, Allen E, Uauy R. Adherence to a physical activity intervention among older adults in a post-transitional middle income country: A quantitative and qualitative analysis. J. Nutr. Health Aging. 2013;17(5):466-471. doi: 10.1007/s12603-012-0417-1. PMID: 23636549 / PMCID: PMC5388178. ISSN: 1279-7707. IF [JCR]: 5.285. Q2
-Resumen: Objectives: The effectiveness of community level interventions depends to a great extent on adherence. Currently, information on factors related to adherence in older adults from developing countries is scarce. Our aim was to identify factors associated to adherence to a physical activity intervention in older adults from a post-transitional middle income country.
Design, setting and participants: Using a combination of quantitative and qualitative methods we studied 996 older Chilean subjects (65-67.9 years at baseline) with low to medium socioeconomic status from 10 health centers randomized to receive a physical activity intervention as part of the CENEX cluster trial (ISRCTN48153354). Measurements: Using a multilevel regression model, the relationship between adherence (defined a priori as attendance at a minimum of 24 physical activity classes spread over at least 12 months) and individual, intervention-related and contextual factors was evaluated. We also conducted 40 semi-structured interviews with older adults (n=36) and instructors (n=4). Transcripts of the interviews were analyzed using content analysis to identify barriers and facilitators to adherence.
Results: Adherence to physical activity intervention was 42.6% (CI 95% 39.5 to 45.6). Depression, diabetes mellitus, percentage of impoverished households and rate of arrests for violent crimes in the neighborhood predicted less adherence (p<0.05) while being retired, participation in physical activity prior to the intervention, and green areas per habitant were positively associated with adherence (p<0.05). The qualitative interviews identified three primary barriers to adherence: current health problems, lack of time due to commitments for caring for family members, and being employed, and two primary facilitators to adherence: the health benefits attributed to the intervention and the opportunity the classes provided for social interaction with others.
Conclusion: In order to enhance effectiveness of community exercise interventions, strategies to improve participation should be targeted to older adults from deprived areas and those with psychological and medical conditions.
Reyes, Marcela
Adipogenic effect of calcium sensing receptor activation. Artículo de revista
En: Mol. Cell. Biochem., 2013.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_199,
title = {Adipogenic effect of calcium sensing receptor activation.},
author = {Marcela Reyes},
doi = {10.1007/s11010-013-1791-7},
year = {2013},
date = {2013-00-00},
urldate = {2013-00-00},
journal = {Mol. Cell. Biochem.},
abstract = {- Otros autores: Villarroel P, Fuentes C, Segovia MP, Tobar N, Villalobos E, Martínez J, Hugo E, Ben-Jonathan N, Cifuentes M.
- Cómo citar: Villarroel P, Fuentes C, Segovia MP, Tobar N, Villalobos E, Martínez J, Hugo E, Ben-Jonathan N, Cifuentes M. Adipogenic effect of calcium sensing receptor activation. Mol. Cell. Biochem. 2013;384(1-2):139-145. doi: 10.1007/s11010-013-1791-7. PMID: 24005534. ISSN: 0300-8177. IF [JCR]: 3.842. Q3
-Resumen: We established that human adipose cells and the human adipose cell line LS14 express the calcium-sensing receptor (CaSR) and that its activation induces inflammatory cytokine production. Also, its expression is enhanced upon exposure to obesity-associated proinflammatory cytokines. We have thus proposed that CaSR activation may be associated with adipose dysfunction. Here, we evaluated a possible effect on adipogenesis. We induced adipose differentiation of primary and LS14 human preadipocytes with or without the simultaneous activation of CaSR, by the exposure to the calcimimetic cinacalcet. Activation of the receptor for 24 h decreased by 40 % the early differentiation marker CCAAT/enhancer-binding protein β. However, upon longer-term (10 day) exposure to the adipogenic cocktail, cinacalcet exerted the opposite effect, causing a dose-response increase in the expression of the mature adipose markers adipocyte protein 2, adiponectin, peroxisome proliferator-activated receptor γ, fatty acid synthase, and glycerol-3-phosphate dehydrogenase. To assess whether there was a time-sensitive effect of CaSR activation on adipogenesis, we evaluated the 10 day effect of cinacalcet exposure for the first 6, 24, 48 h, 6, and 10 days. Our observations suggest that regardless of the period of exposure, 10 day adipogenesis is elevated by cinacalcet. CaSR activation may interfere with the initial stages of adipocyte differentiation; however, these events do not seem to preclude adipogenesis from continuing. Even though adipogenesis (particularly in subcutaneous depots) is associated with insulin sensitivity and adequate adipose function, the implications of our findings in visceral adipocytes, especially in the context of inflamed AT and overnutrition, remain to be established.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Villarroel P, Fuentes C, Segovia MP, Tobar N, Villalobos E, Martínez J, Hugo E, Ben-Jonathan N, Cifuentes M.
- Cómo citar: Villarroel P, Fuentes C, Segovia MP, Tobar N, Villalobos E, Martínez J, Hugo E, Ben-Jonathan N, Cifuentes M. Adipogenic effect of calcium sensing receptor activation. Mol. Cell. Biochem. 2013;384(1-2):139-145. doi: 10.1007/s11010-013-1791-7. PMID: 24005534. ISSN: 0300-8177. IF [JCR]: 3.842. Q3
-Resumen: We established that human adipose cells and the human adipose cell line LS14 express the calcium-sensing receptor (CaSR) and that its activation induces inflammatory cytokine production. Also, its expression is enhanced upon exposure to obesity-associated proinflammatory cytokines. We have thus proposed that CaSR activation may be associated with adipose dysfunction. Here, we evaluated a possible effect on adipogenesis. We induced adipose differentiation of primary and LS14 human preadipocytes with or without the simultaneous activation of CaSR, by the exposure to the calcimimetic cinacalcet. Activation of the receptor for 24 h decreased by 40 % the early differentiation marker CCAAT/enhancer-binding protein β. However, upon longer-term (10 day) exposure to the adipogenic cocktail, cinacalcet exerted the opposite effect, causing a dose-response increase in the expression of the mature adipose markers adipocyte protein 2, adiponectin, peroxisome proliferator-activated receptor γ, fatty acid synthase, and glycerol-3-phosphate dehydrogenase. To assess whether there was a time-sensitive effect of CaSR activation on adipogenesis, we evaluated the 10 day effect of cinacalcet exposure for the first 6, 24, 48 h, 6, and 10 days. Our observations suggest that regardless of the period of exposure, 10 day adipogenesis is elevated by cinacalcet. CaSR activation may interfere with the initial stages of adipocyte differentiation; however, these events do not seem to preclude adipogenesis from continuing. Even though adipogenesis (particularly in subcutaneous depots) is associated with insulin sensitivity and adequate adipose function, the implications of our findings in visceral adipocytes, especially in the context of inflamed AT and overnutrition, remain to be established.
- Cómo citar: Villarroel P, Fuentes C, Segovia MP, Tobar N, Villalobos E, Martínez J, Hugo E, Ben-Jonathan N, Cifuentes M. Adipogenic effect of calcium sensing receptor activation. Mol. Cell. Biochem. 2013;384(1-2):139-145. doi: 10.1007/s11010-013-1791-7. PMID: 24005534. ISSN: 0300-8177. IF [JCR]: 3.842. Q3
-Resumen: We established that human adipose cells and the human adipose cell line LS14 express the calcium-sensing receptor (CaSR) and that its activation induces inflammatory cytokine production. Also, its expression is enhanced upon exposure to obesity-associated proinflammatory cytokines. We have thus proposed that CaSR activation may be associated with adipose dysfunction. Here, we evaluated a possible effect on adipogenesis. We induced adipose differentiation of primary and LS14 human preadipocytes with or without the simultaneous activation of CaSR, by the exposure to the calcimimetic cinacalcet. Activation of the receptor for 24 h decreased by 40 % the early differentiation marker CCAAT/enhancer-binding protein β. However, upon longer-term (10 day) exposure to the adipogenic cocktail, cinacalcet exerted the opposite effect, causing a dose-response increase in the expression of the mature adipose markers adipocyte protein 2, adiponectin, peroxisome proliferator-activated receptor γ, fatty acid synthase, and glycerol-3-phosphate dehydrogenase. To assess whether there was a time-sensitive effect of CaSR activation on adipogenesis, we evaluated the 10 day effect of cinacalcet exposure for the first 6, 24, 48 h, 6, and 10 days. Our observations suggest that regardless of the period of exposure, 10 day adipogenesis is elevated by cinacalcet. CaSR activation may interfere with the initial stages of adipocyte differentiation; however, these events do not seem to preclude adipogenesis from continuing. Even though adipogenesis (particularly in subcutaneous depots) is associated with insulin sensitivity and adequate adipose function, the implications of our findings in visceral adipocytes, especially in the context of inflamed AT and overnutrition, remain to be established.
Garmendia, María Luisa
En: Rev. Medica Chile., 2013.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_30,
title = {Prevención secundaria post infarto agudo de miocardio en hospitales públicos: implementación y resultados de las garantías GES [Universal health coverage and accomplishment of secondary prevention goals among patients with acute myocardial infarction].},
author = {María Luisa Garmendia},
doi = {10.4067/S0034-98872013000800003},
year = {2013},
date = {2013-00-00},
urldate = {2013-00-00},
journal = {Rev. Medica Chile.},
abstract = {- Otros autores: Nazzal C, Lanas F, Bugueño C, Mercadal E, Garcés E, Yovaniniz P, Sanhueza P.
- Cómo citar: Nazzal C, Lanas F, Bugueño C, Mercadal E, Garcés E, Yovaniniz P, Sanhueza P. Prevención secundaria post infarto agudo de miocardio en hospitales públicos: implementación y resultados de las garantías GES [Universal health coverage and accomplishment of secondary prevention goals among patients with acute myocardial infarction]. Rev. Medica Chile. 2013;141(8):977-86. doi: 10.4067/S0034-98872013000800003. PMID: 24448853. ISSN: 0034-9887. IF [JCR]: 0.553. Q4
- Resumen: In 2005, acute myocardial infarction (AMI) was included in a universal health plan (GES) to reduce inequity in care and optimize its diagnosis and treatment. Aim: To evaluate the effect of GES in risk factor control and therapeutic management among patients with AMI. Material and Methods: A survey was conducted in 2008-2009 in six public hospitals. Patients were identified from a hospital based registry of AMI and evaluated one year later with laboratory tests and an interview. Results: The registry enrolled 534 patients with ST and non ST segment elevation myocardial infarction. Of these, 416 patients aged 63 ± 12 years (25% women) were evaluated one year later. Eighty three percent were evaluated by a cardiologist and 37% by a general practitioner. Twenty two percent were evaluated by a nurse and 22% by a nutritionist. At the moment of the interview, 9% smoked, 78% were overweight or obese, 24% performed moderate or vigorous physical activity ≥ 150 min/week, 60% had systolic pressure > 130 mmHg and 63% a diastolic pressure > 80 mmHg. In 30%, LDL cholesterol was > 100 mg/dl and in 43%, triglycerides were > 150 mm/dl. Twenty two percent were diabetic and among them, 52% had a glycosilated hemoglobin > 7%. Forty five percent of non-diabetic patients had a fasting glucose > 100 mg/dl. Ninety three percent were in treatment with aspirin, 86% with statins, 66% with b-blockers, and 73% with angiotensin converting enzyme inhibitors or angiotensin receptor blockers and 20% with clopidogrel. Conclusions: Despite the high proportion of patients in treatment with evidence-based therapy, many do not achieve the targets for risk factor control with the new health care model.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Nazzal C, Lanas F, Bugueño C, Mercadal E, Garcés E, Yovaniniz P, Sanhueza P.
- Cómo citar: Nazzal C, Lanas F, Bugueño C, Mercadal E, Garcés E, Yovaniniz P, Sanhueza P. Prevención secundaria post infarto agudo de miocardio en hospitales públicos: implementación y resultados de las garantías GES [Universal health coverage and accomplishment of secondary prevention goals among patients with acute myocardial infarction]. Rev. Medica Chile. 2013;141(8):977-86. doi: 10.4067/S0034-98872013000800003. PMID: 24448853. ISSN: 0034-9887. IF [JCR]: 0.553. Q4
- Resumen: In 2005, acute myocardial infarction (AMI) was included in a universal health plan (GES) to reduce inequity in care and optimize its diagnosis and treatment. Aim: To evaluate the effect of GES in risk factor control and therapeutic management among patients with AMI. Material and Methods: A survey was conducted in 2008-2009 in six public hospitals. Patients were identified from a hospital based registry of AMI and evaluated one year later with laboratory tests and an interview. Results: The registry enrolled 534 patients with ST and non ST segment elevation myocardial infarction. Of these, 416 patients aged 63 ± 12 years (25% women) were evaluated one year later. Eighty three percent were evaluated by a cardiologist and 37% by a general practitioner. Twenty two percent were evaluated by a nurse and 22% by a nutritionist. At the moment of the interview, 9% smoked, 78% were overweight or obese, 24% performed moderate or vigorous physical activity ≥ 150 min/week, 60% had systolic pressure > 130 mmHg and 63% a diastolic pressure > 80 mmHg. In 30%, LDL cholesterol was > 100 mg/dl and in 43%, triglycerides were > 150 mm/dl. Twenty two percent were diabetic and among them, 52% had a glycosilated hemoglobin > 7%. Forty five percent of non-diabetic patients had a fasting glucose > 100 mg/dl. Ninety three percent were in treatment with aspirin, 86% with statins, 66% with b-blockers, and 73% with angiotensin converting enzyme inhibitors or angiotensin receptor blockers and 20% with clopidogrel. Conclusions: Despite the high proportion of patients in treatment with evidence-based therapy, many do not achieve the targets for risk factor control with the new health care model.
- Cómo citar: Nazzal C, Lanas F, Bugueño C, Mercadal E, Garcés E, Yovaniniz P, Sanhueza P. Prevención secundaria post infarto agudo de miocardio en hospitales públicos: implementación y resultados de las garantías GES [Universal health coverage and accomplishment of secondary prevention goals among patients with acute myocardial infarction]. Rev. Medica Chile. 2013;141(8):977-86. doi: 10.4067/S0034-98872013000800003. PMID: 24448853. ISSN: 0034-9887. IF [JCR]: 0.553. Q4
- Resumen: In 2005, acute myocardial infarction (AMI) was included in a universal health plan (GES) to reduce inequity in care and optimize its diagnosis and treatment. Aim: To evaluate the effect of GES in risk factor control and therapeutic management among patients with AMI. Material and Methods: A survey was conducted in 2008-2009 in six public hospitals. Patients were identified from a hospital based registry of AMI and evaluated one year later with laboratory tests and an interview. Results: The registry enrolled 534 patients with ST and non ST segment elevation myocardial infarction. Of these, 416 patients aged 63 ± 12 years (25% women) were evaluated one year later. Eighty three percent were evaluated by a cardiologist and 37% by a general practitioner. Twenty two percent were evaluated by a nurse and 22% by a nutritionist. At the moment of the interview, 9% smoked, 78% were overweight or obese, 24% performed moderate or vigorous physical activity ≥ 150 min/week, 60% had systolic pressure > 130 mmHg and 63% a diastolic pressure > 80 mmHg. In 30%, LDL cholesterol was > 100 mg/dl and in 43%, triglycerides were > 150 mm/dl. Twenty two percent were diabetic and among them, 52% had a glycosilated hemoglobin > 7%. Forty five percent of non-diabetic patients had a fasting glucose > 100 mg/dl. Ninety three percent were in treatment with aspirin, 86% with statins, 66% with b-blockers, and 73% with angiotensin converting enzyme inhibitors or angiotensin receptor blockers and 20% with clopidogrel. Conclusions: Despite the high proportion of patients in treatment with evidence-based therapy, many do not achieve the targets for risk factor control with the new health care model.
2012
Reyes, Marcela
Antilipolytic effect of calcimimetics depends on the allelic variant of calcium-sensing receptor gene polymorphism rs1042636 (Arg990Gly). Artículo de revista
En: Eur. J. Hum. Gen., 2012.
Resumen | Enlaces | Etiquetas: Otros. /
@article{nokey_210,
title = {Antilipolytic effect of calcimimetics depends on the allelic variant of calcium-sensing receptor gene polymorphism rs1042636 (Arg990Gly).},
author = {Marcela Reyes},
doi = {10.1038/ejhg.2011.221},
year = {2012},
date = {2012-00-00},
urldate = {2012-00-00},
journal = {Eur. J. Hum. Gen.},
abstract = {- Otros autores: Rothe HM, Mattar P, Shapiro WB, Cifuentes M.
- Cómo citar: Rothe HM, Mattar P, Shapiro WB, Cifuentes M. Antilipolytic effect of calcimimetics depends on the allelic variant of calcium-sensing receptor gene polymorphism rs1042636 (Arg990Gly). Eur. J. Hum. Gen. 2012;20(4):480-482. doi: 10.1038/ejhg.2011.221. PMID: 22166946 / PMCID: PMC3306864. ISSN: 1018-4813. IF [JCR]: 5.351. Q1
-Resumen: Calcium-sensing receptor polymorphism rs1042636 (Arg990Gly) affects the response to the calcimimetic cinacalcet, used to treat hypercalcemia in secondary hyperparathyroidism (sHPT) or parathyroid carcinoma. Carriers of the Arg allelle, show less parathyroid hormone secretion suppression in response to the drug. This effect was reproducible in transfected cultured human embryonic kidney cells, supporting a causal relationship on the protein level. We previously established that cinacalcet has an antilipolytic effect in isolated human adipocytes; however, there were a number of samples that did not respond to the treatment. The present work aimed to investigate whether the variable antilipolytic response to cinacalcet in adipocytes was consistent with the effect reported for the rs1042636 polymorphism. Lipolysis was assessed by measuring glycerol release after exposure to cinacalcet (10 μ M) or vehicle in adipocytes isolated from 38 donors. Responsiveness was defined as lipolysis suppression (cinacalcet vs vehicle control) greater than 20%. Genotype analysis showed that 23 adipocyte donors were homozygous for Arg at position 990, 14 heterozygous and 1 homozygous Gly–Gly. Among the Arg homozygotes, one was responsive to cinacalcet, whereas five Gly carriers responded to the calcimimetic. In all, 83% of adipocytes showing response to cinacalcet carried the glycine allele, whereas in 96% of Arg–Arg individuals adipocytes did not respond to the calcimimetic (P=0.027, Fisher's exact test). Confirming sHPT observations, adipocytes from rs1042636 Gly-allele carriers show higher sensitivity to the antilipolytic action of cinacalcet. The potential benefit of cinacalcet as a suppressor of basal lipolysis and free fatty acid release in uremic patients needs to consider the rs1042636 single-nucleotide polymorphism.},
keywords = { Otros. / },
pubstate = {published},
tppubtype = {article}
}
- Otros autores: Rothe HM, Mattar P, Shapiro WB, Cifuentes M.
- Cómo citar: Rothe HM, Mattar P, Shapiro WB, Cifuentes M. Antilipolytic effect of calcimimetics depends on the allelic variant of calcium-sensing receptor gene polymorphism rs1042636 (Arg990Gly). Eur. J. Hum. Gen. 2012;20(4):480-482. doi: 10.1038/ejhg.2011.221. PMID: 22166946 / PMCID: PMC3306864. ISSN: 1018-4813. IF [JCR]: 5.351. Q1
-Resumen: Calcium-sensing receptor polymorphism rs1042636 (Arg990Gly) affects the response to the calcimimetic cinacalcet, used to treat hypercalcemia in secondary hyperparathyroidism (sHPT) or parathyroid carcinoma. Carriers of the Arg allelle, show less parathyroid hormone secretion suppression in response to the drug. This effect was reproducible in transfected cultured human embryonic kidney cells, supporting a causal relationship on the protein level. We previously established that cinacalcet has an antilipolytic effect in isolated human adipocytes; however, there were a number of samples that did not respond to the treatment. The present work aimed to investigate whether the variable antilipolytic response to cinacalcet in adipocytes was consistent with the effect reported for the rs1042636 polymorphism. Lipolysis was assessed by measuring glycerol release after exposure to cinacalcet (10 μ M) or vehicle in adipocytes isolated from 38 donors. Responsiveness was defined as lipolysis suppression (cinacalcet vs vehicle control) greater than 20%. Genotype analysis showed that 23 adipocyte donors were homozygous for Arg at position 990, 14 heterozygous and 1 homozygous Gly–Gly. Among the Arg homozygotes, one was responsive to cinacalcet, whereas five Gly carriers responded to the calcimimetic. In all, 83% of adipocytes showing response to cinacalcet carried the glycine allele, whereas in 96% of Arg–Arg individuals adipocytes did not respond to the calcimimetic (P=0.027, Fisher's exact test). Confirming sHPT observations, adipocytes from rs1042636 Gly-allele carriers show higher sensitivity to the antilipolytic action of cinacalcet. The potential benefit of cinacalcet as a suppressor of basal lipolysis and free fatty acid release in uremic patients needs to consider the rs1042636 single-nucleotide polymorphism.
- Cómo citar: Rothe HM, Mattar P, Shapiro WB, Cifuentes M. Antilipolytic effect of calcimimetics depends on the allelic variant of calcium-sensing receptor gene polymorphism rs1042636 (Arg990Gly). Eur. J. Hum. Gen. 2012;20(4):480-482. doi: 10.1038/ejhg.2011.221. PMID: 22166946 / PMCID: PMC3306864. ISSN: 1018-4813. IF [JCR]: 5.351. Q1
-Resumen: Calcium-sensing receptor polymorphism rs1042636 (Arg990Gly) affects the response to the calcimimetic cinacalcet, used to treat hypercalcemia in secondary hyperparathyroidism (sHPT) or parathyroid carcinoma. Carriers of the Arg allelle, show less parathyroid hormone secretion suppression in response to the drug. This effect was reproducible in transfected cultured human embryonic kidney cells, supporting a causal relationship on the protein level. We previously established that cinacalcet has an antilipolytic effect in isolated human adipocytes; however, there were a number of samples that did not respond to the treatment. The present work aimed to investigate whether the variable antilipolytic response to cinacalcet in adipocytes was consistent with the effect reported for the rs1042636 polymorphism. Lipolysis was assessed by measuring glycerol release after exposure to cinacalcet (10 μ M) or vehicle in adipocytes isolated from 38 donors. Responsiveness was defined as lipolysis suppression (cinacalcet vs vehicle control) greater than 20%. Genotype analysis showed that 23 adipocyte donors were homozygous for Arg at position 990, 14 heterozygous and 1 homozygous Gly–Gly. Among the Arg homozygotes, one was responsive to cinacalcet, whereas five Gly carriers responded to the calcimimetic. In all, 83% of adipocytes showing response to cinacalcet carried the glycine allele, whereas in 96% of Arg–Arg individuals adipocytes did not respond to the calcimimetic (P=0.027, Fisher's exact test). Confirming sHPT observations, adipocytes from rs1042636 Gly-allele carriers show higher sensitivity to the antilipolytic action of cinacalcet. The potential benefit of cinacalcet as a suppressor of basal lipolysis and free fatty acid release in uremic patients needs to consider the rs1042636 single-nucleotide polymorphism.